Influence of titanium ion on mineral formation and properties of osteoid nodules in rat calvaria cultures
Article first published online: 13 AUG 1999
Copyright © 1999 John Wiley & Sons, Inc.
Journal of Biomedical Materials Research
Volume 47, Issue 2, pages 220–227, November 1999
How to Cite
Liao, H., Wurtz, T. and Li, J. (1999), Influence of titanium ion on mineral formation and properties of osteoid nodules in rat calvaria cultures. J. Biomed. Mater. Res., 47: 220–227. doi: 10.1002/(SICI)1097-4636(199911)47:2<220::AID-JBM12>3.0.CO;2-9
- Issue published online: 13 AUG 1999
- Article first published online: 13 AUG 1999
- Manuscript Accepted: 30 MAR 1999
- Manuscript Received: 11 JAN 1999
- calvaria cells;
- osteoblast phenotype;
- titanium ion
The effect of the addition of titanium ion (Ti) on osteoblast function and the mineralization of osteoid nodules in rat calvaria cultures was characterized. Concentrations of 10 ppm of Ti or more inhibited cell proliferation; 5 ppm or less either had no effect or stimulated proliferation. The number of nodules formed was not influenced by 5 ppm of Ti, but mineral deposition in nodules was suppressed, as revealed by von Kossa staining. Likewise, 5 ppm of Ti inhibited the incorporation of [45Ca] in cultures during nodule formation even if the Ti was withdrawn from the medium when mineralization was initiated. In order to test whether the synthesis of osteoid components was affected, the expression of osteonectin (OSN), osteopontin (OPN), osteocalcin (OSC), and alkaline phosphatase (ALP) mRNAs as well as ALP enzyme activity was analyzed. The expression of OSN and OPN mRNAs was reduced dramatically, but OSC mRNA was little affected by 5 ppm of Ti. Ti delayed the development of ALP mRNA expression and enzyme activity relative to the controls. Thus Ti treatment changed the proportional composition of cellular mRNA contributing the osteoblast phenotype. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 47, 220–227, 1999.