Evidence suggesting a stimulatory role for interleukin-10 in erythropoiesis in vitro
Article first published online: 6 DEC 1998
Copyright © 1996 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 166, Issue 2, pages 305–310, February 1996
How to Cite
Wang, C. Q., Udupa, K. B. and Lipschitz, D. A. (1996), Evidence suggesting a stimulatory role for interleukin-10 in erythropoiesis in vitro. J. Cell. Physiol., 166: 305–310. doi: 10.1002/(SICI)1097-4652(199602)166:2<305::AID-JCP8>3.0.CO;2-T
- Issue published online: 6 DEC 1998
- Article first published online: 6 DEC 1998
- Manuscript Accepted: 28 JUL 1995
- Manuscript Received: 16 SEP 1994
- National Institute on Aging, NIH. Grant Number: RO1 AG 09458
- Arkansas EPSCOR
- Dept. of Veterans Affairs
Interleukin-10 (IL-10) has been shown to exert anti-inflammatory effects by suppressing macrophage proliferation and inhibiting cytokine production. In this study we show that in the presence of erythropoietin (EPO), the addition of IL-10 results in a significant dose-dependent increase in both Burst Forming Unit-Erythroid (BFU-E) and Colony Forming Unit-Erythroid (CFU-E) colony growth in both serum-containing and serum-free murine cultures in vitro. IL-10 acts at the later stages of erythroid cell proliferation and differentiation as the increase in colony number was greater in CFU-E than in BFU-E, and was similar when IL-10 was added to BFU-E cultures at the time of culture initiation as when its addition to culture was delayed for 7 days. Furthermore, no increase in BFU-E colony number was noted when IL-10, added at the time of culture initiation, was neutralized by the addition to culture of a monoclonal anti-IL-10 antibody up to 7 days later. The increases in BFU-E by IL-10 addition were not the result of prolongation of BFU-E colony lifespan, which was not significantly different in IL-10 treated and control cultures, respectively. Rather IL-10 stimulated the proliferation of erythroid clusters that were now large enough to be recognized as colonies. IL-10-induced stimulation of erythropoiesis appeared to be independent of its inhibitory effects on macrophage function, as stimulation of erythroid colony growth was similar in macrophage-containing and depleted cultures. Studies to determine if the IL-10 effect was direct or indirect yielded equivocal results. A limiting dilution assay suggested a direct effect. However, a log/log dose response curve with IL-10 did not pass through the origin suggesting an indirect effect. These studies indicate that IL-10 acts synergistically with EPO to significantly increase stimulation of erythroid differentiation and proliferation in vitro and may be involved in the regulation of normal erythropoiesis in vivo. © 1996 Wiley-Liss, Inc.