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Activation of protein kinase A is a pivotal step involved in both BMP-2- and cyclic AMP-induced chondrogenesis
Article first published online: 6 DEC 1998
DOI: 10.1002/(SICI)1097-4652(199702)170:2<153::AID-JCP7>3.0.CO;2-N
Copyright © 1997 Wiley-Liss, Inc.
1097-4652/asset/cover.gif?v=1&s=23a74af39293034ea4f4c2517aa3cfc422028b41 "Journal of Cellular Physiology")
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How to Cite
Lee, Y.-S. and Chuong, C.-M. (1997), Activation of protein kinase A is a pivotal step involved in both BMP-2- and cyclic AMP-induced chondrogenesis. Journal of Cellular Physiology, 170: 153–165. doi: 10.1002/(SICI)1097-4652(199702)170:2<153::AID-JCP7>3.0.CO;2-N
Publication History
- Issue published online: 6 DEC 1998
- Article first published online: 6 DEC 1998
- Manuscript Accepted: 20 SEP 1996
- Manuscript Received: 23 JAN 1996
Funded by
- NIH
- NSF
- CTR
- Abstract
- References
- Cited By
Abstract
We studied the roles of protein kinase A (PKA) activation and cyclic AMP response element binding protein (CREB) phosphorylation in chondrogenesis using serum-free chicken limb bud micromass cultures as a model system. We showed the following points: (1) in micromass cultures, activation of PKA enhances chondrogenesis and increases the phosphorylation of CREB; (2) BMP-2, a chondrogenic stimulator, increases PKA activity and the level of phosphorylated CREB (P-CREB); (3) H8, a PKA inhibitor, inhibits chondrogenesis; (4) the chondrogenic activities of BMP-2 and cAMP are suppressed by H8; and (5) long-term TPA treatment (a protein kinase C (PKC) modulator) inhibits chondrogenesis and decreases the levels of CREB and P-CREB. These results suggest that activation of PKA is a physiological event during chondrogenesis that is involved in the chondrogenic effects of both BMP-2 and cyclic AMP (cAMP)-dependent pathways. J. Cell. Physiol. 170:153–165, 1997. © 1997 Wiley-Liss, Inc.