Genetics, Gene Regulation, and Expression

Specific expression of soluble adenylyl cyclase in male germ cells

  1. Meeghan L. Sinclair1,
  2. Xiang-Yuan Wang2,
  3. Melissa Mattia1,
  4. Marco Conti3,
  5. Jochen Buck1,
  6. Debra J. Wolgemuth2,
  7. Lonny R. Levin1,*

Article first published online: 22 MAR 2000

DOI: 10.1002/(SICI)1098-2795(200005)56:1<6::AID-MRD2>3.0.CO;2-M

Issue

Molecular Reproduction and Development

Molecular Reproduction and Development

Volume 56, Issue 1, pages 6–11, May 2000

Additional Information

How to Cite

Sinclair, M. L., Wang, X.-Y., Mattia, M., Conti, M., Buck, J., Wolgemuth, D. J. and Levin, L. R. (2000), Specific expression of soluble adenylyl cyclase in male germ cells. Molecular Reproduction and Development, 56: 6–11. doi: 10.1002/(SICI)1098-2795(200005)56:1<6::AID-MRD2>3.0.CO;2-M

Author Information

  1. 1

    Department of Pharmacology, Weill Medical College of Cornell University, New York, New York

  2. 2

    Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York

  3. 3

    Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

*Department of Pharmacology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10021.

Publication History

  1. Issue published online: 22 MAR 2000
  2. Article first published online: 22 MAR 2000
  3. Manuscript Accepted: 18 NOV 1999
  4. Manuscript Received: 24 SEP 1999

Funded by

  • National Institutes of Health. Grant Numbers: DK48022, DK52797, GM52891, DA07274, HD34915, HD31544

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Keywords:

  • testis;
  • cAMP;
  • in situ hybridization;
  • spermatogenesis;
  • pachytene spermatocytes;
  • spermatids

Abstract

The cAMP signaling pathway is an important mediator of extracellular signals in organisms from prokaryotes to higher eukaryotes. In mammals two types of adenylyl cyclase synthesize cAMP; a ubiquitous family of transmembrane isoforms regulated by G proteins in response to extracellular signals, and a recently isolated soluble enzyme insensitive to heterotrimeric G protein modulation. Using the very sensitive reverse transcription-polymerase chain reaction (RT-PCR), soluble adenylyl cyclase (sAC) expression is detectable in almost all tissues examined; however, Northern analysis and in situ hybridization indicate that high levels of sAC message are unique to male germ cells. Elevated levels of sAC mRNA are first observed in pachytene spermatocytes and expression increases through spermiogenesis. The accumulation of high levels of message in round spermatids suggests sAC protein plays an important role in the generation of cAMP in spermatozoa, implying possible roles in sperm maturation through the epididymis, capacitation, hypermotility, and/or the acrosome reaction. Mol. Reprod. Dev. 56:6–11, 2000. © 2000 Wiley-Liss, Inc.

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