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Keywords:

  • Dyes;
  • Fluorescence spectroscopy;
  • Nitrogen monoxide;
  • Sensors;
  • Signal transduction

Abstract

The membrane-permeating indicator DAF-FM DA is transformed by intracellular esterases into the highly water-soluble dye DAF-FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location of NO.