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Keywords:

  • Antigen-specific T lymphocyte;
  • Cytokine-secretion;
  • Memory T cell;
  • Affinity matrix technology;
  • Magnetic cell sorting

Abstract

Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HLA-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.