Techniques in genomic imprinting research
Part 1. Genetics
Basic Techniques and Approaches
Published Online: 15 NOV 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Gray, T. A. 2005. Techniques in genomic imprinting research. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 1:1.3:44.
- Published Online: 15 NOV 2005
Contemporary assays to evaluate candidate-imprinted genes are varied, but they generally fall into two categories: those that assess monoallelic expression and those that assess DNA methylation. Parental origin-dependent expression is the most definitive test for imprinted gene determination. Alternatively, cytosine DNA methylation is a measurable epigenetic feature that correlates with gene transcription and thus commonly serves as a DNA-based indicator of monoallelic expression. Outlined below are approaches that examine both differential expression by RT-PCR of transcribed polymorphisms, and differential cytosine methylation by amplification of bisulfite-converted genomic DNA.
- bisulfite conversion;
- differential transcription;
- differential methylation;
- genomic sequencing;
- methylation-specific PCR