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Techniques in genomic imprinting research

Part 1. Genetics

1.3. Epigenetics

Basic Techniques and Approaches

  1. Todd A. Gray

Published Online: 15 NOV 2005

DOI: 10.1002/047001153X.g103409

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Gray, T. A. 2005. Techniques in genomic imprinting research. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 1:1.3:44.

Author Information

  1. Wadsworth Center, The Genomics Institute, Troy, NY, USA

Publication History

  1. Published Online: 15 NOV 2005

Abstract

Contemporary assays to evaluate candidate-imprinted genes are varied, but they generally fall into two categories: those that assess monoallelic expression and those that assess DNA methylation. Parental origin-dependent expression is the most definitive test for imprinted gene determination. Alternatively, cytosine DNA methylation is a measurable epigenetic feature that correlates with gene transcription and thus commonly serves as a DNA-based indicator of monoallelic expression. Outlined below are approaches that examine both differential expression by RT-PCR of transcribed polymorphisms, and differential cytosine methylation by amplification of bisulfite-converted genomic DNA.

Keywords:

  • bisulfite conversion;
  • differential transcription;
  • differential methylation;
  • epigenetic;
  • genomic sequencing;
  • methylation-specific PCR