Control of transgene expression in mammalian cells
Part 1. Genetics
1.7. Gene Therapy
Basic Techniques and Approaches
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Kramer, B. P. and Fussenegger, M. 2005. Control of transgene expression in mammalian cells. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 1:1.7:104.
- Published Online: 15 JAN 2005
Spacio-temporal adjustment of therapeutic transgene expression represents a fundamental technology for modern gene therapy since it enables precise in vivo dosing of protein therapeutics to manage an optimal therapeutic range, adaptation to specific disease dynamics, or resetting of molecular interventions as appropriate. Heterologous transcription control systems capitalizing on (1) prokaryotic antibiotic response regulons evolved to manage bacterial adaptation to macrolide, streptogramin, and tetracycline antibiotics, (2) (modified) hormone receptors specific for estradiol, progesterone, or ecdysone derivatives, or (3) chemically induced dimerization of protein domains prevailed in animal studies, and are currently considered for clinical trials. Since sustained administration of clinically licensed, small-molecule drugs for transgene expression fine-tuning may result in side effects, systems responsive to nucleic acids or physical parameters including light, temperature, and electromagnetic fields are in preclinical development.
- heterologous gene regulation;
- inducible/repressible promoter;