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Sequencing templates – shotgun clone isolation versus amplification approaches

Part 2. Genomics

2.1. Genome Sequencing

Specialist Review

  1. Rebecca Deadman,
  2. Carl W. Fuller

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g201206

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Deadman, R. and Fuller, C. W. 2005. Sequencing templates – shotgun clone isolation versus amplification approaches. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.1:4.

Author Information

  1. GE Healthcare, Piscataway, NJ, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Preparation of good-quality DNA is essential for obtaining high-quality DNA sequence data. Alkaline lysis remains the most popular method for isolating small and large DNA constructs, but new techniques including solid-phase immobilization and rolling circle amplification offer alternatives to the traditional approaches. Quality, quantity, and consistency of isolated DNA vary from method to method, and the choice of DNA isolation method should be carefully considered, particularly when a variety of vectors or host strains will be used.

Keywords:

  • plasmid;
  • sequencing;
  • BAC;
  • DNA purification;
  • alkaline lysis;
  • rolling circle amplification