Restriction fragment fingerprinting software
Part 2. Genomics
Short Specialist Review
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Soderlund, C. A. 2005. Restriction fragment fingerprinting software. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.2:19.
- Published Online: 15 JAN 2005
A physical map is an ordering of clones and markers. Whole-genome physical maps may be constructed using restriction fragment fingerprinting. With this approach, two clones are considered overlapping if they contain a similar set of fragments. The FPC (FingerPrinted Contig) software is described, which assembles fingerprinted clones into contigs. Markers can also be used to aid the assembly and results in a partial ordering of the markers. Genetic or radiation hybrid markers are used to anchor the contigs to the chromosomes. A minimal tiling path of clones may be selected for sequencing. FPC may be used with the two salient types of fingerprinting, which are agarose based and HICF (High Information Content Fingerprinting).
- restriction fragment;
- genetic markers;
- minimal tiling path