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Restriction fragment fingerprinting software

Part 2. Genomics

2.2. Mapping

Short Specialist Review

  1. Carol A. Soderlund

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g202312

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Soderlund, C. A. 2005. Restriction fragment fingerprinting software. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.2:19.

Author Information

  1. University of Arizona, Tucson, AZ, USA

Publication History

  1. Published Online: 15 JAN 2005

Abstract

A physical map is an ordering of clones and markers. Whole-genome physical maps may be constructed using restriction fragment fingerprinting. With this approach, two clones are considered overlapping if they contain a similar set of fragments. The FPC (FingerPrinted Contig) software is described, which assembles fingerprinted clones into contigs. Markers can also be used to aid the assembly and results in a partial ordering of the markers. Genetic or radiation hybrid markers are used to anchor the contigs to the chromosomes. A minimal tiling path of clones may be selected for sequencing. FPC may be used with the two salient types of fingerprinting, which are agarose based and HICF (High Information Content Fingerprinting).

Keywords:

  • mapping;
  • restriction fragment;
  • fingerprinting;
  • assembly;
  • markers;
  • genetic markers;
  • sequencing;
  • minimal tiling path