Environmental shotgun sequencing
Part 2. Genomics
2.5. Bacteria and Other Pathogens
Short Specialist Review
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Tyson, G. W. and Hugenholtz, P. 2005. Environmental shotgun sequencing. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.5:65.
- Published Online: 15 APR 2005
Shotgun sequencing of microbial communities is a promising means for delving into community function and interactions, population structure and dynamics, and bypassing the cultivation bottleneck in microbiology. However, this approach is mostly blind to low-abundance species as it effectively samples only the dominant populations in a community. It also faces a number of technical challenges such as the assembly and separation of individual genomes from a mixture of genomic fragments compounded by sampling unevenness and data scalability. Despite these limitations, environmental shotgun sequencing should rapidly advance the fields of microbial ecology and evolution.
- microbial ecology and evolution;
- shotgun sequencing;
- genome assembly