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Technologies for systematic analysis of eukaryotic transcriptomes

Part 2. Genomics

2.7. ESTs: Cancer Genes and the Anatomy Project

Introductory Review

  1. Robert L. Strausberg

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g207107

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Strausberg, R. L. 2005. Technologies for systematic analysis of eukaryotic transcriptomes. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:79.

Author Information

  1. The J. Craig Venter Institute, Rockville, MD, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Comprehensive high-throughput analysis of the eukaryotic transcriptome provides unprecedented opportunity to understand gene structure and gene expression, and to use that knowledge to gain perspective of biological function. During the past decade, several new technologies based on DNA sequencing and hybridization approaches have been developed and applied to transcriptome analysis. In this overview, applications of these current technologies are discussed, as is the need for continued technology development.

Keywords:

  • gene expression;
  • cDNA;
  • SAGE;
  • MPSS;
  • microarray;
  • genome