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What is an EST?

Part 2. Genomics

2.7. ESTs: Cancer Genes and the Anatomy Project

Introductory Review

  1. Winston A. Hide1,
  2. Andrey Ptitsyn2

Published Online: 15 NOV 2005

DOI: 10.1002/047001153X.g207108

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Hide, W. A. and Ptitsyn, A. 2005. What is an EST?. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:78.

Author Information

  1. 1

    South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa

  2. 2

    Pennington Biomedical Research Center, Baton Rouge, LA, USA

Publication History

  1. Published Online: 15 NOV 2005

Abstract

An expressed sequence tag (EST) is the name given to a sequence of a randomly picked complementary DNA sequence (cDNA) derived from processing messenger RNA from an mRNA library with a reverse transcriptase.

ESTs represent the first truly high-throughput technology to have populated the databases and have made the rapid growth of advanced computational studies in biology inevitable. They have marked the transition of modern biology from genetics to the genomics era. ESTs offer the first glimpse of the transcriptome: that volume of transcripts copied from genes and forwarded to all compartments of a living cell as it functions.

Keywords:

  • EST;
  • expressed sequence tags;
  • EST manufacture;
  • transcript diversity;
  • shotgun sequencing