What is an EST?
Part 2. Genomics
2.7. ESTs: Cancer Genes and the Anatomy Project
Published Online: 15 NOV 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Hide, W. A. and Ptitsyn, A. 2005. What is an EST?. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:78.
- Published Online: 15 NOV 2005
An expressed sequence tag (EST) is the name given to a sequence of a randomly picked complementary DNA sequence (cDNA) derived from processing messenger RNA from an mRNA library with a reverse transcriptase.
ESTs represent the first truly high-throughput technology to have populated the databases and have made the rapid growth of advanced computational studies in biology inevitable. They have marked the transition of modern biology from genetics to the genomics era. ESTs offer the first glimpse of the transcriptome: that volume of transcripts copied from genes and forwarded to all compartments of a living cell as it functions.
- expressed sequence tags;
- EST manufacture;
- transcript diversity;
- shotgun sequencing