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Using ESTs for genome annotation – predicting the transcriptome

Part 2. Genomics

2.7. ESTs: Cancer Genes and the Anatomy Project

Specialist Review

  1. Eduardo Eyras

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g207206

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Eyras, E. 2005. Using ESTs for genome annotation – predicting the transcriptome. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:81.

Author Information

  1. Pompeu Fabra University - IMIM, Barcelona, Spain

Publication History

  1. Published Online: 15 JUL 2005

Abstract

Expressed sequence tags (ESTs) provide a quick and cost-effective way to obtain information about the collection of expressed genes in a genome. They can also provide information about the expression of genes, alternative splicing, polymorphisms, and untranslated regions, complementing other standard methods of annotation and ab initio gene prediction. Here we describe some of the advantages and problems of the usage of ESTs for genome annotation, emphasizing their utility for describing alternative splicing.

Keywords:

  • genomics;
  • automatic annotation;
  • expressed sequences;
  • EST;
  • alternative splicing