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Pilot gene discovery in plasmodial pathogens

Part 2. Genomics

2.7. ESTs: Cancer Genes and the Anatomy Project

Short Specialist Review

  1. Jane M. Carlton

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g207304

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Carlton, J. M. 2005. Pilot gene discovery in plasmodial pathogens. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:86.

Author Information

  1. The Institute for Genomic Research, Rockville, MD, USA

Publication History

  1. Published Online: 15 JAN 2005

Abstract

Before the onset and completion of the Plasmodium genome sequencing projects, gene discovery in species of malaria parasite relied heavily upon pilot EST sequencing projects. Stage-specific cDNA libraries were generated for several human and rodent model malaria parasite species, and partial sequencing of individual cDNA clones enabled the production of gene tags that could then be searched against public sequence databases for gene identification and annotation. A wealth of partial gene sequence data was generated and made available to the scientific community in order to jump-start gene discovery and biological investigation. The importance of these efforts has been reemphasized recently with the publication of the genome sequences of two Plasmodium species that relied upon the EST data for training gene finder software and for gene model verification. The generation of Plasmodium EST data continues and provides invaluable gene expression data for use in a postgenomic world.

Keywords:

  • expressed sequence tag;
  • Plasmodium;
  • malaria;
  • parasite;
  • gene tag;
  • stage-specific;
  • transcript;
  • gene discovery