Pilot gene discovery in plasmodial pathogens
Part 2. Genomics
2.7. ESTs: Cancer Genes and the Anatomy Project
Short Specialist Review
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Carlton, J. M. 2005. Pilot gene discovery in plasmodial pathogens. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:86.
- Published Online: 15 JAN 2005
Before the onset and completion of the Plasmodium genome sequencing projects, gene discovery in species of malaria parasite relied heavily upon pilot EST sequencing projects. Stage-specific cDNA libraries were generated for several human and rodent model malaria parasite species, and partial sequencing of individual cDNA clones enabled the production of gene tags that could then be searched against public sequence databases for gene identification and annotation. A wealth of partial gene sequence data was generated and made available to the scientific community in order to jump-start gene discovery and biological investigation. The importance of these efforts has been reemphasized recently with the publication of the genome sequences of two Plasmodium species that relied upon the EST data for training gene finder software and for gene model verification. The generation of Plasmodium EST data continues and provides invaluable gene expression data for use in a postgenomic world.
- expressed sequence tag;
- gene tag;
- gene discovery