Standard Article

Manufacturing EST libraries

Part 2. Genomics

2.7. ESTs: Cancer Genes and the Anatomy Project

Basic Techniques and Approaches

  1. Marcelo B. Soares,
  2. Maria F. Bonaldo

Published Online: 15 NOV 2005

DOI: 10.1002/047001153X.g207416

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Soares, M. B. and Bonaldo, M. F. 2005. Manufacturing EST libraries. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.7:87.

Author Information

  1. Northwestern University, Children's Memorial Research Center, Chicago, IL, USA

Publication History

  1. Published Online: 15 NOV 2005

Abstract

Here we address problems that occur systematically during the manufacturing of EST libraries, and that have an impact on the analysis and interpretation of EST data. We discuss their cause, consequences, and diagnostic features.

Keywords:

  • cDNA library;
  • expressed sequence tag (EST);
  • EST library;
  • gene discovery;
  • transcriptome analysis;
  • first-strand cDNA synthesis;
  • artifactual cDNAs;
  • EST analysis