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Microarray CGH

Part 2. Genomics

2.8. Expression Profiling

Specialist Review

  1. Denis A. Smirnov1,
  2. Vivian G. Cheung2

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g208201

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Smirnov, D. A. and Cheung, V. G. 2005. Microarray CGH. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.8:93.

Author Information

  1. 1

    Immunicon Corporation, Huntington Valley, PA, USA

  2. 2

    University of Pennsylvania, Philadelphia, PA, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Comparative genomic hybridization (CGH) is a molecular cytogenetic method for detection of chromosomal abnormalities. Traditionally, normal metaphase chromosomes were used as substrates for CGH analysis. Recent advances in microarray technology and developments in the human genome project allow substitution of metaphase chromosomes with collections of arrayed genomic clones, cDNA clones, or oligonucleotides. When combined with such microarrays, CGH can be used for genome-wide high-resolution characterization of cytogenetic imbalances. This review focuses on recent advances and remaining challenges in manufacturing genomic microarrays and using them for identification of chromosomal abnormalities by microarray CGH.

Keywords:

  • comparative genomic hybridization;
  • CGH;
  • array CGH;
  • genomic microarrays;
  • genetic diagnostics;
  • chromosomal imbalances;
  • microarray