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Expression and localization of proteins in mammalian cells

Part 2. Genomics

2.8. Expression Profiling

Specialist Review

  1. Jennifer L. Stow,
  2. Rohan D. Teasdale

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g208202

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Stow, J. L. and Teasdale, R. D. 2005. Expression and localization of proteins in mammalian cells. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.8:94.

Author Information

  1. The University of Queensland, Brisbane, Australia

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The transfer of genes and expression of recombinant proteins is one of the main methods for discovery in the quest for understanding as biology moves from genomes through to proteomes, and finally to working cells and organisms. The techniques and tools for the transfer and engineering of genetic material and for the detection and analysis of expressed proteins are evolving rapidly alongside the burgeoning databases they have to explore. Physical, chemical, and biologically based vectors increasingly enable gene transfer into mammalian cells for research and therapeutic purposes. The emergence of fluorescent protein tags now allows sophisticated tracking and functional analysis of expressed proteins. In this review, we summarize the methods and scientific tools contributing to this technology. Finally, the push is on to develop high-throughput and computational methods for large-scale protein expression, localization, and analysis, and here we set the stage by providing updates on the coming technology in these fields.

Keywords:

  • transfection;
  • protein expression;
  • subcellular localization;
  • high-throughput screening;
  • green fluorescent protein;
  • bioinformatics