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Bacterial genome organization: comparative expression profiling, operons, regulons, and beyond

Part 2. Genomics

2.8. Expression Profiling

Short Specialist Review

  1. Scott N. Peterson1,2

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g208309

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Peterson, S. N. 2005. Bacterial genome organization: comparative expression profiling, operons, regulons, and beyond. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.8:98.

Author Information

  1. 1

    The Institute for Genomic Research, Rockville, MD, USA

  2. 2

    The George Washington University, Washington, DC, USA

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The sequencing of complete microbial genomes has provided a molecular basis for the diversity of bacterial species. Bacterial gene expression in response to environmental stimuli may be equally diverse and raises the question as to how well what we learn about gene expression in one species translates to another. A problem common to all scientists performing global gene expression lies in determining the significance of gene expression changes as they relate to the functional requirements of the encoded proteins in the biological process under investigation. Four recent studies are presented that highlight some of the issues that confound direct interpretation of gene expression data, including operon structure, functional redundancy of proteins, and the occurrence of nonorthologous genes in bacteria. The concept of comparative expression profiling (CEP) is introduced and discussed in the context of the experimental results observed.

Keywords:

  • microbial;
  • genomics;
  • comparative expression profiling;
  • operons;
  • DNA microarrays;
  • gene expression;
  • regulons;
  • genome organization;
  • non-orthologous genes