The use of external controls in microarray experiments
Part 2. Genomics
2.8. Expression Profiling
Basic Techniques and Approaches
Published Online: 15 OCT 2004
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
van Helvoort, J. M., van Leenen, D. and Holstege, F. C. 2004. The use of external controls in microarray experiments. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 2:2.8:102.
- Published Online: 15 OCT 2004
Accuracy in reporting expression levels or differences therein is an important issue for microarray technology. Here we discuss the reasons for using external RNA controls for the optimization of nearly every aspect of microarray technology. The main conclusion is that external RNA controls offer an extremely versatile system that does not suffer from the disadvantages of other approaches and also lend themselves for assessing accuracy within every subsequent microarray experiment after optimization. We describe what external controls are, how they can contribute toward systematic analysis of accuracy, and how such controls should be implemented. Other uses of external controls such as normalization, determining sensitivity, and reporting absolute mRNA copies per cell are also discussed.
- external controls;
- spike-in controls;
- mRNA copy per cell;
- expression profiling;