Advances of LC-MS and CZE-MS in proteome analysis
Part 3. Proteomics
3.1. Core Methodologies
Published Online: 15 APR 2006
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Lee, H. and Aebersold, R. 2006. Advances of LC-MS and CZE-MS in proteome analysis. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1.
- Published Online: 15 APR 2006
The combination of high-performance separation techniques and mass spectrometry (MS) has had a significant impact on proteomics over the past decade. High-resolution separation of peptides prior to MS is essential to improve sensitivity, speed, and throughput of the analysis of peptides that originate from the global digestion of complex protein mixtures. This article will review recent advances of microcapillary chromatography and capillary zone electrophoresis (CZE) in the field of proteomics.
- microcapillary liquid chromatography;
- capillary zone electrophoresis;
- mass spectrometry;
- electrospray ionization