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Advances of LC-MS and CZE-MS in proteome analysis

Part 3. Proteomics

3.1. Core Methodologies

Introductory Review

  1. Hookeun Lee1,
  2. Ruedi Aebersold1,2

Published Online: 15 APR 2006

DOI: 10.1002/047001153X.g301108

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Lee, H. and Aebersold, R. 2006. Advances of LC-MS and CZE-MS in proteome analysis. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1.

Author Information

  1. 1

    Institute for Molecular Systems Biology, ETH Zürich, Switzerland

  2. 2

    University of Zürich, Zürich, Switzerland

Publication History

  1. Published Online: 15 APR 2006

Abstract

The combination of high-performance separation techniques and mass spectrometry (MS) has had a significant impact on proteomics over the past decade. High-resolution separation of peptides prior to MS is essential to improve sensitivity, speed, and throughput of the analysis of peptides that originate from the global digestion of complex protein mixtures. This article will review recent advances of microcapillary chromatography and capillary zone electrophoresis (CZE) in the field of proteomics.

Keywords:

  • microcapillary liquid chromatography;
  • capillary zone electrophoresis;
  • mass spectrometry;
  • proteomics;
  • electrospray ionization