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Sample preparation for proteomics

Part 3. Proteomics

3.1. Core Methodologies

Specialist Review

  1. Thierry Rabilloud

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g301212

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Rabilloud, T. 2005. Sample preparation for proteomics. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:2.

Author Information

  1. CEA-Laboratoire d'Immunochimie, Grenoble, France

Publication History

  1. Published Online: 15 APR 2005

Abstract

Various types of sample preparation for downstream proteomics analysis are outlined. The rationales for native or denaturing extractions are discussed, together with the constraints brought by the protein or peptide separation methods, that is, one- or two-dimensional denaturing protein electrophoresis, native protein electrophoresis, or peptide chromatography. The constraints discussed in this chapter also include the removal of nonprotein substances interfering with the proteomics analysis.

Keywords:

  • protein solubilization;
  • protein denaturation;
  • disulfide bonds;
  • chaotropes;
  • electrophoresis;
  • two-dimensional electrophoresis;
  • proteolysis;
  • peptide chromatography