Sample preparation for proteomics
Part 3. Proteomics
3.1. Core Methodologies
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Rabilloud, T. 2005. Sample preparation for proteomics. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:2.
- Published Online: 15 APR 2005
Various types of sample preparation for downstream proteomics analysis are outlined. The rationales for native or denaturing extractions are discussed, together with the constraints brought by the protein or peptide separation methods, that is, one- or two-dimensional denaturing protein electrophoresis, native protein electrophoresis, or peptide chromatography. The constraints discussed in this chapter also include the removal of nonprotein substances interfering with the proteomics analysis.
- protein solubilization;
- protein denaturation;
- disulfide bonds;
- two-dimensional electrophoresis;
- peptide chromatography