Standard Article

Time-of-flight mass spectrometry

Part 3. Proteomics

3.1. Core Methodologies

Short Specialist Review

  1. Robert J. Cotter

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g301302

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Cotter, R. J. 2005. Time-of-flight mass spectrometry. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:7.

Author Information

  1. Johns Hopkins University School of Medicine, Baltimore, MD, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Time-of-flight (TOF) mass spectrometers have become the instruments of choice where high molecular masses must be measured. Pulsed extraction, orthogonal extraction, and reflectron designs have greatly improved mass resolution. In addition, hybrid and tandem instruments, such as quadrupole/TOF, ion trap/TOF, and TOF/TOF mass spectrometers now provide the opportunity for obtaining tandem mass spectra. Time-of-flight instruments are used in proteomics for determining the molecular weights of intact proteins, for accurate mass measurements of tryptic peptides (mass fingerprinting), for amino acid sequencing (sequence tags) and for determining the locations and structures of posttranslational modifications.

Keywords:

  • mass resolution;
  • spatial distribution;
  • kinetic energy distribution;
  • reflectron;
  • electron ionization (EI);
  • matrix-assisted laser desorption/ionization (MALDI);
  • time-to-digital converter (TDC);
  • pulsed extraction;
  • transient recorder;
  • channel-plate detector