Standard Article

Multidimensional liquid chromatography tandem mass spectrometry for biological discovery

Part 3. Proteomics

3.1. Core Methodologies

Short Specialist Review

  1. Claire M. Delahunty,
  2. John R. Yates III

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g301317

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Delahunty, C. M. and Yates, J. R. 2005. Multidimensional liquid chromatography tandem mass spectrometry for biological discovery. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:13.

Author Information

  1. The Scripps Research Institute, LaJolla, CA, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Biological systems organize physiological functions and communications into collections of proteins. Multidimensional liquid chromatography (MudPIT) in combination with tandem mass spectrometry can be used to identify proteins in such structures. This data reveals potential roles for proteins in the context of the structures.

Keywords:

  • proteomics;
  • MudPIT;
  • 2D liquid chromatography;
  • protein identification