Tutorial on tandem mass spectrometry database searching
Part 3. Proteomics
3.1. Core Methodologies
Basic Techniques and Approaches
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Eng, J. K. 2005. Tutorial on tandem mass spectrometry database searching. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics.
- Published Online: 15 APR 2005
Identifying peptides, and ultimately proteins, by searching sequence databases with tandem mass spectra of peptides is one of the most widely used proteomics techniques. The steps by which a user actually performs a search are fairly straightforward and are described in this article. Although this article goes through the identification process of searching a single tandem mass spectrum to identify a single peptide, most users will not usually selective search only one spectrum but would query the full set of tandem mass spectra acquired in an entire LC-MS/MS run. The underlying steps and analysis considerations outlined here are relevant in either scenario.
- database searching;
- peptide fingerprinting;
- tandem mass spectrometry;