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Handling membrane proteins

Part 3. Proteomics

3.1. Core Methodologies

Basic Techniques and Approaches

  1. Robert J. A. Goode1,2,
  2. Richard J. Simpson1

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g301415

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Goode, R. J. A. and Simpson, R. J. 2005. Handling membrane proteins. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:15.

Author Information

  1. 1

    Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Joint proteomics Laboratory (JPSL), Parkville, Victoria, Australia

  2. 2

    The University of Melbourne, Department of Biochemistry and Molecular Biology, Parkville, Victoria, Australia

Publication History

  1. Published Online: 15 APR 2005

Abstract

Membrane proteins fulfill several vital roles, including cell adhesion, solute transport, and receptor functions. Despite these important biological functions, very little is known about the structure and expression of these proteins at the protein level due to their low abundance, inherent hydrophobicity, and variable posttranslational modifications. In order to overcome these obstacles, classical proteomic techniques have been adapted and several techniques have been developed, allowing characterization of elusive membrane proteins.

Keywords:

  • membranes;
  • membrane proteins;
  • cell surface;
  • detergents;
  • cell fractionation/methods;
  • electrophoresis