Handling membrane proteins
Part 3. Proteomics
3.1. Core Methodologies
Basic Techniques and Approaches
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Goode, R. J. A. and Simpson, R. J. 2005. Handling membrane proteins. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:15.
- Published Online: 15 APR 2005
Membrane proteins fulfill several vital roles, including cell adhesion, solute transport, and receptor functions. Despite these important biological functions, very little is known about the structure and expression of these proteins at the protein level due to their low abundance, inherent hydrophobicity, and variable posttranslational modifications. In order to overcome these obstacles, classical proteomic techniques have been adapted and several techniques have been developed, allowing characterization of elusive membrane proteins.
- membrane proteins;
- cell surface;
- cell fractionation/methods;