Standard Article

Techniques for ion dissociation (fragmentation) and scanning in MS/MS

Part 3. Proteomics

3.1. Core Methodologies

Basic Techniques and Approaches

  1. Jack Throck Watson1,
  2. O. David Sparkman2

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g301416

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Watson, J. T. and Sparkman, O. D. 2005. Techniques for ion dissociation (fragmentation) and scanning in MS/MS. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:18.

Author Information

  1. 1

    Michigan State University, East Lansing, MI, USA

  2. 2

    University of the Pacific, Stockton, CA, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

A determination of the mass of an ion, although important, may be insufficient for identification (see Mass spectrometry, FT-ICR, Quadrupole MS, and Quadrupole Ion Traps and a New Era of Evolution). Often, the manner in which and/or the degree to which an ion can be made to dissociate provides a wealth of characteristic and structural information. There are different instrumental approaches to promote and conduct ion dissociation, and there are several different “scanning” modes to obtain and utilize the ion dissociation information.

Keywords:

  • collisionally-activated dissociation (CAD);
  • mass spectrometry/mass spectrometry (MS/MS);
  • structure clucidation;
  • neutral-loss scan;
  • precursor ion