Techniques for ion dissociation (fragmentation) and scanning in MS/MS
Part 3. Proteomics
3.1. Core Methodologies
Basic Techniques and Approaches
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Watson, J. T. and Sparkman, O. D. 2005. Techniques for ion dissociation (fragmentation) and scanning in MS/MS. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:18.
- Published Online: 15 APR 2005
A determination of the mass of an ion, although important, may be insufficient for identification (see Mass spectrometry, FT-ICR, Quadrupole MS, and Quadrupole Ion Traps and a New Era of Evolution). Often, the manner in which and/or the degree to which an ion can be made to dissociate provides a wealth of characteristic and structural information. There are different instrumental approaches to promote and conduct ion dissociation, and there are several different “scanning” modes to obtain and utilize the ion dissociation information.
- collisionally-activated dissociation (CAD);
- mass spectrometry/mass spectrometry (MS/MS);
- structure clucidation;
- neutral-loss scan;
- precursor ion