Making nanocolumns and tips
Part 3. Proteomics
3.1. Core Methodologies
Basic Techniques and Approaches
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Delahunty, C. and Yates, J. R. 2005. Making nanocolumns and tips. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.1:19.
- Published Online: 15 JAN 2005
This tutorial describes the construction of fritless nanocolumns and tips from 100-µm microcapillary tubing. Protocols are given for pulling the tips using a commercial laser puller as well as for packing a single-phase or multidimensional column. The nanocolumns described are for use in in-line µLC separations and are typically coupled to an ESI source.
- mass spectrometry