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Separation-dependent approaches for protein expression profiling

Part 3. Proteomics

3.2. Expression Proteomics

Introductory Review

  1. Michael J. Dunn,
  2. Stephen R. Pennington

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g302108

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Dunn, M. J. and Pennington, S. R. 2005. Separation-dependent approaches for protein expression profiling. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:20.

Author Information

  1. Proteome Research Centre, University College Dublin, Conway Institute for Biomolecular and Biomedical Research, Dublin, Ireland

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The depth and breadth of genomic data now available for a wide variety of prokaryotic and eukaryotic organisms provides a previously unimagined opportunity to perform comprehensive profiling studies of patterns of protein expression in complex biological systems that will result in new insights into cellular functions that determine biologically relevant phenotypes. In this chapter, we give an introduction to protein expression profiling in which the individual proteins in a complex sample are separated prior to semiquantitative analysis. We outline the alternative approaches based either on the use of one- and two-dimensional gel electrophoresis or on the application of so-called gel-free methods based on the use of liquid chromatography coupled either on- or off-line to mass spectrometry.

Keywords:

  • expression profiling;
  • image analysis;
  • liquid chromatography;
  • mass spectrometry;
  • peptide mass fingerprinting;
  • protein expression;
  • protein identification;
  • proteomics;
  • stable isotope labeling;
  • two-dimensional gel electrophoresis