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Separation-independent approaches for protein expression profiling

Part 3. Proteomics

3.2. Expression Proteomics

Introductory Review

  1. Rosalind E. Jenkins1,
  2. Stephen R. Pennington2

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g302110

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Jenkins, R. E. and Pennington, S. R. 2005. Separation-independent approaches for protein expression profiling. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:21.

Author Information

  1. 1

    University of Liverpool, Liverpool, UK

  2. 2

    Proteome Research Centre, University College Dublin, Conway Institute for Biomolecular and Biomedical Research, Dublin, Ireland

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The field of proteomics has expanded enormously in the last few years, but many of the methods used to investigate proteins are based on relatively old technologies such as two-dimensional gel electrophoresis. While such approaches have been extremely useful in protein expression mapping, and still generate useful data today, global coverage of protein expression, activity, and protein interactions both temporally and spatially will require the continued development of higher-throughput approaches. The analysis of gene expression has been revolutionized by the advent of DNA chip technology, and it is now becoming apparent that a similar approach would be invaluable for the analysis of the dynamic proteome. This article introduces the concept of protein and antibody chips and, by comparison with nucleic acid–based microarrays, highlights the practical issues to be addressed if this technology is to become widely applicable.

Keywords:

  • protein microarray;
  • antibody microarray;
  • high-throughput screening