Separation-independent approaches for protein expression profiling
Part 3. Proteomics
3.2. Expression Proteomics
Published Online: 15 JUL 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Jenkins, R. E. and Pennington, S. R. 2005. Separation-independent approaches for protein expression profiling. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:21.
- Published Online: 15 JUL 2005
The field of proteomics has expanded enormously in the last few years, but many of the methods used to investigate proteins are based on relatively old technologies such as two-dimensional gel electrophoresis. While such approaches have been extremely useful in protein expression mapping, and still generate useful data today, global coverage of protein expression, activity, and protein interactions both temporally and spatially will require the continued development of higher-throughput approaches. The analysis of gene expression has been revolutionized by the advent of DNA chip technology, and it is now becoming apparent that a similar approach would be invaluable for the analysis of the dynamic proteome. This article introduces the concept of protein and antibody chips and, by comparison with nucleic acid–based microarrays, highlights the practical issues to be addressed if this technology is to become widely applicable.
- protein microarray;
- antibody microarray;
- high-throughput screening