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ICAT and other labeling strategies for semiquantitative LC-based expression profiling

Part 3. Proteomics

3.2. Expression Proteomics

Specialist Review

  1. Connie Byrne,
  2. Gerard Cagney

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g302201

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Byrne, C. and Cagney, G. 2005. ICAT and other labeling strategies for semiquantitative LC-based expression profiling. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:23.

Author Information

  1. University College Dublin, Dublin, Ireland

Publication History

  1. Published Online: 15 APR 2005

Abstract

Comprehensive proteome analysis requires efficient, robust, and practical methods for the identification and quantification of all proteins in complex biological samples. Mass spectrometry is a well-established protein identification tool and semiquantitative proteomics has traditionally been performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. However, other separation strategies based on liquid chromatography now complement 2-DE as a central method for analysis of proteins in complex biological systems. In this review, we discuss the most promising methodological developments in this area with particular attention to their merits and limitations.

Keywords:

  • liquid chromatography;
  • mass spectrometry;
  • expression profiling strategies;
  • proteomics;
  • in vivo labeling;
  • in vitro labeling