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Protein arrays

Part 3. Proteomics

3.2. Expression Proteomics

Specialist Review

  1. Derek Murphy,
  2. Dolores J. Cahill

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g302203

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Murphy, D. and Cahill, D. J. 2005. Protein arrays. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:24.

Author Information

  1. Royal College of Surgeons in Ireland, Centre for Human Proteomics, Dublin, Ireland

Publication History

  1. Published Online: 15 JUL 2005

Abstract

Protein array technology is becoming an increasingly important tool in the drive toward proteome-scale analysis of protein activity and interactions. Presently, this technology compliments the more traditional methods for proteomic analysis, including two-dimensional gel electrophoresis/chromatography and mass spectrometry. While the task of producing a “whole-proteome” chip, containing active proteins, is a daunting one, current protein and antibody arrays represent the first steps toward that goal. In this review, we discuss current approaches for the generation of protein arrays, and their applications, including their use in the study of protein–protein, protein–nucleic acid, enzyme–substrate, and so on, interactions. Potential applications of protein arrays in interaction screening, such as compound–protein interactions are also discussed.

Keywords:

  • protein array;
  • chip microarray;
  • antibody;
  • surfaces;
  • peptide screening;
  • autoimmune;
  • allergen;
  • diagnostics;
  • drug discovery;
  • interaction studies