Part 3. Proteomics
3.2. Expression Proteomics
Short Specialist Review
Published Online: 15 OCT 2004
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Lilley, K. S. 2004. 2D DIGE. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:25.
- Published Online: 15 OCT 2004
Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) (see 2-DE) has been the major separations techniques used in proteomics, allowing the resolution of several thousand proteins in a single sample. The limitations of this technique have been low sensitivity, reduced dynamic range, and gel-to-gel variability. Difference gel electrophoresis (DIGE) circumvents the issues associated with traditional 2D PAGE and allows more accurate and sensitive quantitative proteomics studies.
- 2D PAGE;
- minimal CyDyes;
- saturation CyDyes