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2D DIGE

Part 3. Proteomics

3.2. Expression Proteomics

Short Specialist Review

  1. Kathryn S. Lilley

Published Online: 15 OCT 2004

DOI: 10.1002/047001153X.g302312

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Lilley, K. S. 2004. 2D DIGE. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:25.

Author Information

  1. University of Cambridge, Cambridge, UK

Publication History

  1. Published Online: 15 OCT 2004

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) (see 2-DE) has been the major separations techniques used in proteomics, allowing the resolution of several thousand proteins in a single sample. The limitations of this technique have been low sensitivity, reduced dynamic range, and gel-to-gel variability. Difference gel electrophoresis (DIGE) circumvents the issues associated with traditional 2D PAGE and allows more accurate and sensitive quantitative proteomics studies.

Keywords:

  • DIGE;
  • 2D PAGE;
  • minimal CyDyes;
  • saturation CyDyes