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Arraying proteins and antibodies

Part 3. Proteomics

3.2. Expression Proteomics

Basic Techniques and Approaches

  1. David S. Wilson,
  2. Steffen Nock

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g302402

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Wilson, D. S. and Nock, S. 2005. Arraying proteins and antibodies. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:32.

Author Information

  1. Absalus Inc., Mountain View, CA, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Protein arrays (see Protein microarrays as an emerging tool for proteomics and Protein arrays) enable investigators to characterize multiple proteins in parallel while expending minimal amounts of precious biological samples. The individual features of arrays can be either arranged in a two-dimensional grid on a planar surface, or they can be constructed from optically encoded particles suspended in a solution. A variety of substrates, chemistries, and arraying methods have been described. The choice among these methods should be made according to the nature of the application and the types and numbers of proteins that need to be arrayed.

Keywords:

  • protein array;
  • antibody array;
  • expression profiling;
  • proteomics;
  • multiplexed assay