2-D Difference Gel Electrophoresis – an accurate quantitative method for protein analysis
Part 3. Proteomics
3.2. Expression Proteomics
Basic Techniques and Approaches
Published Online: 15 NOV 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Hawkins, E. and David, S. O. 2005. 2-D Difference Gel Electrophoresis – an accurate quantitative method for protein analysis. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:30.
- Published Online: 15 NOV 2005
2-D Difference Gel Electrophoresis (DIGE) is a novel technique that enables multiple protein extracts to be labeled with different fluorescent dyes. The labeled samples are then separated on the same 2-D gel. The dyes known as CyDyeTM DIGE Fluors are spectrally resolvable as well as size- and charge-matched.
The ability to add more than one sample to a 2-D electrophoresis (2-DE) gel enables the introduction of an internal standard that adds the quantitative advantages of a reference sample commonly seen in DNA microarray studies.
Measurement of protein abundance as a “Standardized Ratio” instead of an “Absolute volume” greatly reduces the influence of experimental variation commonly associated with conventional 2-DE.
- 2-D DIGE;
- CyDyeTM DIGE Fluors;
- internal standard