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MS-based methods for identification of 2-DE-resolved proteins

Part 3. Proteomics

3.2. Expression Proteomics

Basic Techniques and Approaches

  1. Achim Treumann1,
  2. Christopher Gerner2

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g302409

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Treumann, A. and Gerner, C. 2005. MS-based methods for identification of 2-DE-resolved proteins. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:31.

Author Information

  1. 1

    Royal College of Surgeons in Ireland, Dublin, Ireland

  2. 2

    Medical University of Vienna, Vienna, Austria

Publication History

  1. Published Online: 15 APR 2005

Abstract

Separation of proteins by two-dimensional gel electrophoresis followed by mass spectrometric protein identification is one of the main procedural workflows in proteomics. Peptide mass fingerprinting and peptide fragmentation are the two most frequently used applications of mass spectrometry for this purpose. We present an introductory overview and a short discussion of the respective merits of both approaches.

Keywords:

  • 2-DE;
  • mass spectrometry;
  • peptide mass fingerprinting;
  • collision-induced dissociation;
  • peptide fragmentation;
  • sequencing;
  • proteomics