MS-based methods for identification of 2-DE-resolved proteins
Part 3. Proteomics
3.2. Expression Proteomics
Basic Techniques and Approaches
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Treumann, A. and Gerner, C. 2005. MS-based methods for identification of 2-DE-resolved proteins. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:31.
- Published Online: 15 APR 2005
Separation of proteins by two-dimensional gel electrophoresis followed by mass spectrometric protein identification is one of the main procedural workflows in proteomics. Peptide mass fingerprinting and peptide fragmentation are the two most frequently used applications of mass spectrometry for this purpose. We present an introductory overview and a short discussion of the respective merits of both approaches.
- mass spectrometry;
- peptide mass fingerprinting;
- collision-induced dissociation;
- peptide fragmentation;