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Basic techniques for the use of reverse phase protein microarrays for signal pathway profiling

Part 3. Proteomics

3.2. Expression Proteomics

Basic Techniques and Approaches

  1. Virginia Espina1,
  2. Valerie S. Calvert2,
  3. Julia Wulfkuhle1,
  4. Lance A. Liotta1,
  5. Emanuel F. Petricoin III2

Published Online: 15 NOV 2005

DOI: 10.1002/047001153X.g302414

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Espina, V., Calvert, V. S., Wulfkuhle, J., Liotta, L. A. and Petricoin, E. F. 2005. Basic techniques for the use of reverse phase protein microarrays for signal pathway profiling. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.2:33.

Author Information

  1. 1

    National Cancer Institute, Bethesda, MD, USA

  2. 2

    Center for Biologics Evaluation and Research, Bethesda, MD, USA

Publication History

  1. Published Online: 15 NOV 2005

Abstract

Ultimately, disease-related genetic defects are manifested on a protein level, involving derangements in protein function and the information flow within diseased cells and the surrounding tissue microenvironment. New classes of protein microarrays have been recently developed using highly sensitive, specific technology, and can be used to generate a “wiring diagram” of information flow mediated through posttranslational modification driven networks. Through the combined use of laser capture microdissection of pure populations of tumor cells obtained from patient biopsies, these arrays can provide the foundation for the development of individualized combinatorial therapies of molecular inhibitors to target tumor-specific deranged pathways regulating key biologic processes including proliferation, differentiation, apoptosis, immunity, and metastasis. In the future, therapies can be tailored to the specific deranged molecular circuitry of an individual patient's disease. The successful transition of these technologies from research tools to integrated clinical diagnostic platforms will require ongoing continued development, development of reference standards, and optimization with rigorous standardization development and quality-control procedures.

Keywords:

  • proteomics;
  • reverse phase;
  • protein microarray;
  • signal pathway;
  • phosphoproteomics