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The yeast interactome

Part 3. Proteomics

3.3. Mapping of Biochemical Networks

Specialist Review

  1. Peter Uetz1,
  2. Andrei Grigoriev2

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g303204

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Uetz, P. and Grigoriev, A. 2005. The yeast interactome. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.3:39.

Author Information

  1. 1

    Institute of Genetics (ITG), Forschungszentrum Karlsruhe, Karlsruhe, Germany

  2. 2

    GPC Biotech, Martinsried, Germany

Publication History

  1. Published Online: 15 APR 2005

Abstract

The yeast proteome and its interactome (that is, the sum of all protein interactions) are the best studied of all organisms. Currently, there are about 3000 verified protein interactions and several thousand nonverified interactions known in yeast. Independent studies estimated that there may be more than 30 000 interactions in yeast although most estimates rather suggest 15 000–25 000. It remains unknown how many of these interactions are really essential. The average yeast protein appears to have about five interactions but this number may represent an overestimate because many proteins of yet unknown function exhibit fewer interactions. Nevertheless, most proteins can be connected in a huge network of interactions. The protein interaction network of yeast is highly dynamic although there are 1500 or more proteins involved in several hundred stable complexes. The dynamics and regulation of protein interaction networks, for example, by protein modifications, are only now being explored. There are no clear physical properties of a yeast protein that predicts its interaction properties but several protein families (such as proteins involved in RNA splicing) seem to be much more highly connected than others (such as metabolic enzymes).

Keywords:

  • proteomics;
  • network;
  • two-hybrid system;
  • mass spectrometry;
  • interaction map;
  • interactome;
  • yeast;
  • protein complex;
  • synthetic lethality;
  • genetic interaction