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Membrane-anchored protein complexes

Part 3. Proteomics

3.3. Mapping of Biochemical Networks

Short Specialist Review

  1. Igor Stagljar

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g303307

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Stagljar, I. 2005. Membrane-anchored protein complexes. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.3:42.

Author Information

  1. University of Zurich-Irchel, Zurich, Switzerland

Publication History

  1. Published Online: 15 JAN 2005

Abstract

Proteins associated with membranes total approximately one-third of all proteins in a typical eukaryotic cell. Owing to their pivotal role in many cellular processes, their direct link to human disease, and their often extracellular accessibility toward drugs, an understanding of membrane protein function is desirable. However, the hydrophobic nature of membrane proteins often results in insoluble proteins, which makes protein isolation difficult and therefore hinders the determination of protein complex composition and protein function. This article provides a brief overview of the available yeast-based interactive proteomic technologies for detecting protein interactions among membrane proteins.

Keywords:

  • membrane proteins;
  • drug targets;
  • protein–protein interactions;
  • yeast Saccharomyces cerevisiae;
  • genetic assays;
  • G-protein fusion assay;
  • reverse Ras recruitment system;
  • split-ubiquitin membrane yeast two-hybrid systems