Bioluminescence resonance energy transfer
Part 3. Proteomics
3.3. Mapping of Biochemical Networks
Basic Techniques and Approaches
Published Online: 15 NOV 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Jockers, R. and Marullo, S. 2005. Bioluminescence resonance energy transfer. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.3:47.
- Published Online: 15 NOV 2005
Bioluminescence Resonance Energy Transfer (BRET) has become a method of choice to monitor and quantify both constitutive and regulated interactions among proteins in intact cells. Despite the fact that BRET cannot be used to visualize the subcellular compartment where the interaction between two proteins occurs, it offers a number of practical advantages over FRET, including higher sensitivity in microplate-based high-throughput assays. The advantages of BRET-based approaches are illustrated by some significant advances obtained in various fields, such as protein oligomerization, ligand-induced conformational changes, and regulated movement of proteins between subcellular compartments.
- energy transfer;
- protein–protein interaction