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Probing cellular function with bioluminescence imaging

Part 3. Proteomics

3.4. Functional Proteomics

Introductory Review

  1. Guy A. Rutter1,
  2. Gabriela da Silva Xavier1,
  3. Takashi Tsuboi1,
  4. Kathryn J. Mitchell1,
  5. John W. Hanrahan2

Published Online: 15 OCT 2004

DOI: 10.1002/047001153X.g304110

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Rutter, G. A., da Silva Xavier, G., Tsuboi, T., Mitchell, K. J. and Hanrahan, J. W. 2004. Probing cellular function with bioluminescence imaging. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:48.

Author Information

  1. 1

    University of Bristol, Bristol, UK

  2. 2

    McGill University, QC, Canada

Publication History

  1. Published Online: 15 OCT 2004

Abstract

Bioluminescence is widely used by lower organisms as a means for communication. In recent years, gene transfer technologies have allowed this potential to be harnessed by cell biologists interested in intracellular signaling and other cellular processes. Here, we focus on the use of bioluminescence to image ATP, Ca2+, and gene expression in single mammalian cells.

Keywords:

  • bioluminescence;
  • luciferase;
  • aequorin;
  • imaging