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Quantitative EM techniques

Part 3. Proteomics

3.4. Functional Proteomics

Specialist Review

  1. John M. Lucocq

Published Online: 15 OCT 2006

DOI: 10.1002/047001153X.g304217

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Lucocq, J. M. 2006. Quantitative EM techniques. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4.

Author Information

  1. School of Life Sciences, University of Dundee, Dundee, UK

Publication History

  1. Published Online: 15 OCT 2006

Abstract

This article reviews current methods for quantification from profiles of biological structures displayed on thin sections in electron microscopy. These methods use appropriate sampling combined with design-based approaches to estimate the number and sizes of cellular structures or compartments and also to quantify particulate immunolabelling of molecular components.

Keywords:

  • stereology;
  • immunoelectron microscopy;
  • quantitative EM;
  • electron microscopy;
  • thin sections;
  • gold labelling;
  • sampling