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Photobleaching (FRAP/FLIP) and dynamic imaging

Part 3. Proteomics

3.4. Functional Proteomics

Short Specialist Review

  1. George Banting

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g304301

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Banting, G. 2005. Photobleaching (FRAP/FLIP) and dynamic imaging. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:53.

Author Information

  1. University of Bristol, Bristol, UK

Publication History

  1. Published Online: 15 APR 2005

Abstract

The techniques of Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) both provide ways to monitor the movement of fluorescently tagged proteins within live cells. The development of genetically encoded fluorescent tags (e.g., green fluorescent protein, GFP) has made FRAP and FLIP accessible to researchers with a broad range of interests and technical expertise. This review outlines the principles underlying FRAP and FLIP, highlights the type of information that may be obtained using these techniques, and points out some caveats that need to be considered when using them.

Keywords:

  • GFP;
  • FRAP;
  • FLIP;
  • photobleach;
  • fluorophore;
  • live cell;
  • diffusion