Photobleaching (FRAP/FLIP) and dynamic imaging
Part 3. Proteomics
3.4. Functional Proteomics
Short Specialist Review
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Banting, G. 2005. Photobleaching (FRAP/FLIP) and dynamic imaging. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:53.
- Published Online: 15 APR 2005
The techniques of Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) both provide ways to monitor the movement of fluorescently tagged proteins within live cells. The development of genetically encoded fluorescent tags (e.g., green fluorescent protein, GFP) has made FRAP and FLIP accessible to researchers with a broad range of interests and technical expertise. This review outlines the principles underlying FRAP and FLIP, highlights the type of information that may be obtained using these techniques, and points out some caveats that need to be considered when using them.
- live cell;