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Quantum dots for multiparameter measurements

Part 3. Proteomics

3.4. Functional Proteomics

Short Specialist Review

  1. Marcel P. Bruchez

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g304302

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Bruchez, M. P. 2005. Quantum dots for multiparameter measurements. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:57.

Author Information

  1. Quantum Dot Corporation, Hayward, CA, USA

Publication History

  1. Published Online: 15 JUL 2005

Abstract

Quantum dot materials have emerged as powerful alternatives to conventional fluorescent molecules. Their optical properties are perfectly suited to the information-intensive requirements of modern molecular and cellular biology assays, and have enabled a number of new ways of investigating cellular systems. Multicolor labeling with quantum dots allows collection of information on location, distribution, and abundance of multiple proteins in cellular and blot-based systems with ratio-based quantitation. The brightness and photostability of these materials provide researchers the means to investigate protein dynamics and changes in cellular and animal models over physiologically relevant timescales, and in situations where organic fluorescence probes fail. The spectral properties can be used effectively to extend the capability of multiplexed assays using multicolor beads as assay supports that are easily decoded to provide quantitative information on nucleic acids and proteins. A variety of researchers exploit these properties to open new avenues for biological investigation of the action and interactions of many molecules simultaneously.

Keywords:

  • multiplex;
  • multiparameter;
  • quantum dot;
  • fluorescence;
  • assay;
  • detection;
  • imaging;
  • gene expression