Quantum dots for multiparameter measurements
Part 3. Proteomics
3.4. Functional Proteomics
Short Specialist Review
Published Online: 15 JUL 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Bruchez, M. P. 2005. Quantum dots for multiparameter measurements. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics.
- Published Online: 15 JUL 2005
Quantum dot materials have emerged as powerful alternatives to conventional fluorescent molecules. Their optical properties are perfectly suited to the information-intensive requirements of modern molecular and cellular biology assays, and have enabled a number of new ways of investigating cellular systems. Multicolor labeling with quantum dots allows collection of information on location, distribution, and abundance of multiple proteins in cellular and blot-based systems with ratio-based quantitation. The brightness and photostability of these materials provide researchers the means to investigate protein dynamics and changes in cellular and animal models over physiologically relevant timescales, and in situations where organic fluorescence probes fail. The spectral properties can be used effectively to extend the capability of multiplexed assays using multicolor beads as assay supports that are easily decoded to provide quantitative information on nucleic acids and proteins. A variety of researchers exploit these properties to open new avenues for biological investigation of the action and interactions of many molecules simultaneously.
- quantum dot;
- gene expression