Standard Article

Probing protein function in cells using CALI

Part 3. Proteomics

3.4. Functional Proteomics

Short Specialist Review

  1. Thomas J. Diefenbach,
  2. Daniel G. Jay

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g304305

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Diefenbach, T. J. and Jay, D. G. 2005. Probing protein function in cells using CALI. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:54.

Author Information

  1. Tufts University School of Medicine, Boston, MA, USA

Publication History

  1. Published Online: 15 JAN 2005

Abstract

Chromophore-assisted laser inactivation (CALI) is a method utilizing dye-labeled antibodies combined with light of specific wavelengths for acute knockdown of protein function at the cellular and tissue level with high spatial and temporal resolution. In recent years, the techniques of CALI have evolved, although the goal of direct knockdown of the protein function remains the same. This article will examine the utility, applications, and derivations of CALI as a tool to probe in vitro and in vivo protein function.

Keywords:

  • chromophore;
  • laser;
  • inactivation;
  • protein;
  • proteomic;
  • laser;
  • antibody;
  • antibody labeling;
  • protein inactivation;
  • free radicals;
  • malachite green isothiocyanate;
  • fluorescein isothiocyanate