Probing protein function in cells using CALI
Part 3. Proteomics
3.4. Functional Proteomics
Short Specialist Review
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Diefenbach, T. J. and Jay, D. G. 2005. Probing protein function in cells using CALI. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics.
- Published Online: 15 JAN 2005
Chromophore-assisted laser inactivation (CALI) is a method utilizing dye-labeled antibodies combined with light of specific wavelengths for acute knockdown of protein function at the cellular and tissue level with high spatial and temporal resolution. In recent years, the techniques of CALI have evolved, although the goal of direct knockdown of the protein function remains the same. This article will examine the utility, applications, and derivations of CALI as a tool to probe in vitro and in vivo protein function.
- antibody labeling;
- protein inactivation;
- free radicals;
- malachite green isothiocyanate;
- fluorescein isothiocyanate